Composite

Part:BBa_K3385068:Design

Designed by: Lucas Levassor   Group: iGEM20_DTU-Denmark   (2020-10-16)


T1G3R_Spilot_glaA


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1858
    Illegal EcoRI site found at 5765
    Illegal EcoRI site found at 7193
    Illegal XbaI site found at 1623
    Illegal XbaI site found at 5796
    Illegal SpeI site found at 1097
    Illegal PstI site found at 893
    Illegal PstI site found at 4027
    Illegal PstI site found at 6603
    Illegal PstI site found at 8337
    Illegal PstI site found at 8938
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1858
    Illegal EcoRI site found at 5765
    Illegal EcoRI site found at 7193
    Illegal NheI site found at 4904
    Illegal SpeI site found at 1097
    Illegal PstI site found at 893
    Illegal PstI site found at 4027
    Illegal PstI site found at 6603
    Illegal PstI site found at 8337
    Illegal PstI site found at 8938
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1858
    Illegal EcoRI site found at 5765
    Illegal EcoRI site found at 7193
    Illegal BglII site found at 3882
    Illegal BglII site found at 5404
    Illegal BglII site found at 8435
    Illegal BamHI site found at 1729
    Illegal BamHI site found at 2157
    Illegal BamHI site found at 4862
    Illegal BamHI site found at 5664
    Illegal BamHI site found at 5790
    Illegal BamHI site found at 6341
    Illegal XhoI site found at 548
    Illegal XhoI site found at 735
    Illegal XhoI site found at 963
    Illegal XhoI site found at 6201
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1858
    Illegal EcoRI site found at 5765
    Illegal EcoRI site found at 7193
    Illegal XbaI site found at 1623
    Illegal XbaI site found at 5796
    Illegal SpeI site found at 1097
    Illegal PstI site found at 893
    Illegal PstI site found at 4027
    Illegal PstI site found at 6603
    Illegal PstI site found at 8337
    Illegal PstI site found at 8938
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1858
    Illegal EcoRI site found at 5765
    Illegal EcoRI site found at 7193
    Illegal XbaI site found at 1623
    Illegal XbaI site found at 5796
    Illegal SpeI site found at 1097
    Illegal PstI site found at 893
    Illegal PstI site found at 4027
    Illegal PstI site found at 6603
    Illegal PstI site found at 8337
    Illegal PstI site found at 8938
    Illegal NgoMIV site found at 2566
    Illegal NgoMIV site found at 5122
    Illegal NgoMIV site found at 8325
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3410
    Illegal BsaI site found at 3548
    Illegal BsaI site found at 4237
    Illegal BsaI.rc site found at 4730
    Illegal SapI site found at 3097
    Illegal SapI site found at 8826
    Illegal SapI site found at 9135


Design Notes

Full construct made using non-overlapping primers, which can pose difficulties on PCR amplification and subsequently USER assembly.

Source

Sequences were designed using the model SignalPrepper.

References